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Rxr reporter gene assay
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Rxr reporter gene assay


Date:26.05.2016, 20:14 Altogether the results of the present study show that the newly developed, yeast two-hybrid assay can be used as a valuable tool for identification and quantification of compounds active in disturbing retinoid homeostasis at the level of RXR. The 20 relative inhibitory concentration (RIC20) values of r-hexachlorocyclohexane (HCH p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) and 2,4-DCP with metabolization were lower than 1 x 10(-6)mol/L. These results suggest that BPA, HCH, p,p'-DDT and 2,4-DCP are chemicals that pose a threat to hRXR functioning. Agonistic and antagonistic actions of chemicals including a series of phenols, phthalates, organochlorine pesticides (OCPs) were tested in the absence and presence of 5 x 10(-6)mol/L 9-cis RA, at which maximal beta-galactosidase activity could be induced. The aim of this study is to investigate the chemical retinoic acid (RA) disruption at the level of retinoid X receptor (RXR) functioning. This assay makes use of recombined human RXR gene and reporter gene yeast, which specifically expresses beta-galactosidase when incubated with exogenous. The results obtained reveal that some chemicals, e.g., 2-t-butylphenol, 2-isopropylphenol, 2,4-dichlorophenol (2,4-DCP 3,4-dichlorophenol (3,4-DCP 4-tert-octylphenol (4-t-OP) and hexachlorobenzene (HCB are RXR agonists. Especially, bisphenol A (BPA) showed high induction activity to RXR when tested with metabolization.
Rxr reporter gene assay


Rxr reporter gene assay Rxr reporter gene assay


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